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Nycomed ficoll–hypaque gradients lymphoprep
Ficoll–Hypaque Gradients Lymphoprep, supplied by Nycomed, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+lymphoprep/ficoll+hypaque+lymphoprep/pmc11619616-85-16-19
Average 90 stars, based on 1 article reviews
ficoll–hypaque gradients lymphoprep - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Isolation:

Article Title: Mesenchymal precursor cell
Article Snippet: Bone marrow mononuclear cells (BMMNC) were obtained by centrifugation over Ficoll 1.077 g/ml (Lymphoprep, Nycomed, Oslo, Norway) at 400 g for 30 minutes (min) and then washed and resuspended with Hank's buffered saline solution containing 1% bovine serum albumin and 10 mM HEPES, pH 7.35 (HBSS).

Article Title: Inhibition of TIM‐4 protects against cerebral ischaemia‐reperfusion injury
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on a Ficoll (Lymphoprep; PAA; Nycomed) gradient from buffy coat preparations obtained from mice and then washed twice in ice‐cold PBS.

Article Title: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on a Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradient from buffy coat preparations obtained from mice, and then washed twice with PBS.

Article Title: CD123 redirected multiple virus-specific T cells for acute myeloid leukemia.
Article Snippet: Hematopoietic stem cell transplantation (HSCT) has been increasingly used as a curative treatment for acute myeloid leukemia (AML).. However, relapse rates after HSCT in complete remission (CR) are reported between 30% and 70%.. In addition, numerous studies suggested that secondary viral infection from a variety of viruses including Epstein-Barr virus (EBV), adenovirus (Adv), and cytomegalovirus (CMV) are among the most common causes of death post-HSCT.

Article Title: Safety and feasibility of virus-specific T cells derived from umbilical cord blood in cord blood transplant recipients
Article Snippet: CBMCs were washed twice, resuspended in CellGenix media (CellGenix USA, Antioch, IL), and plated at approximately 1 × 10 7 cells per mL in 2 mL of dendritic cell (DC) medium (CellGenix media plus 2 mM L-glutamine) (GlutaMAX; Invitrogen, Carlsbad, CA) in a 6-well plate (Costar, Corning, NY) for 1 to 2 hours at 37°C in a humidified CO 2 incubator.

Article Title: CMV-Specific T-cells Generated From Naïve T-cells Recognize Atypical Epitopes And May Be Protective in Vivo
Article Snippet: CB was thawed and then purified by Ficoll (Lymphoprep; Nycomed) gradient separation.

Article Title: High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Article Snippet: Mononuclear cells were isolated from bone marrow aspirates (30 mL) by density gradient centrifugation in Ficoll (density, 1.077 g/mL) (Lymphoprep, Nycomed Pharma) and plated in noncoated 75–175 cm 2 polystyrene culture flasks (Corning Costar, Celbio, Italy) at a density of 160,000 cells/cm 2 .

Article Title: Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradients from buffy coat preparations obtained from mice, and then washed twice with PBS.

Centrifugation:

Article Title: Mesenchymal precursor cell
Article Snippet: Bone marrow mononuclear cells (BMMNC) were obtained by centrifugation over Ficoll 1.077 g/ml (Lymphoprep, Nycomed, Oslo, Norway) at 400 g for 30 minutes (min) and then washed and resuspended with Hank's buffered saline solution containing 1% bovine serum albumin and 10 mM HEPES, pH 7.35 (HBSS).

Article Title: Inhibition of TIM‐4 protects against cerebral ischaemia‐reperfusion injury
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on a Ficoll (Lymphoprep; PAA; Nycomed) gradient from buffy coat preparations obtained from mice and then washed twice in ice‐cold PBS.

Article Title: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on a Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradient from buffy coat preparations obtained from mice, and then washed twice with PBS.

Article Title: CD123 redirected multiple virus-specific T cells for acute myeloid leukemia.
Article Snippet: Hematopoietic stem cell transplantation (HSCT) has been increasingly used as a curative treatment for acute myeloid leukemia (AML).. However, relapse rates after HSCT in complete remission (CR) are reported between 30% and 70%.. In addition, numerous studies suggested that secondary viral infection from a variety of viruses including Epstein-Barr virus (EBV), adenovirus (Adv), and cytomegalovirus (CMV) are among the most common causes of death post-HSCT.

Article Title: Safety and feasibility of virus-specific T cells derived from umbilical cord blood in cord blood transplant recipients
Article Snippet: CBMCs were washed twice, resuspended in CellGenix media (CellGenix USA, Antioch, IL), and plated at approximately 1 × 10 7 cells per mL in 2 mL of dendritic cell (DC) medium (CellGenix media plus 2 mM L-glutamine) (GlutaMAX; Invitrogen, Carlsbad, CA) in a 6-well plate (Costar, Corning, NY) for 1 to 2 hours at 37°C in a humidified CO 2 incubator.

Article Title: CMV-Specific T-cells Generated From Naïve T-cells Recognize Atypical Epitopes And May Be Protective in Vivo
Article Snippet: CB was thawed and then purified by Ficoll (Lymphoprep; Nycomed) gradient separation.

Article Title: High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Article Snippet: Mononuclear cells were isolated from bone marrow aspirates (30 mL) by density gradient centrifugation in Ficoll (density, 1.077 g/mL) (Lymphoprep, Nycomed Pharma) and plated in noncoated 75–175 cm 2 polystyrene culture flasks (Corning Costar, Celbio, Italy) at a density of 160,000 cells/cm 2 .

Article Title: Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradients from buffy coat preparations obtained from mice, and then washed twice with PBS.

Purification:

Article Title: Mesenchymal precursor cell
Article Snippet: Bone marrow mononuclear cells (BMMNC) were obtained by centrifugation over Ficoll 1.077 g/ml (Lymphoprep, Nycomed, Oslo, Norway) at 400 g for 30 minutes (min) and then washed and resuspended with Hank's buffered saline solution containing 1% bovine serum albumin and 10 mM HEPES, pH 7.35 (HBSS).

Article Title: Inhibition of TIM‐4 protects against cerebral ischaemia‐reperfusion injury
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on a Ficoll (Lymphoprep; PAA; Nycomed) gradient from buffy coat preparations obtained from mice and then washed twice in ice‐cold PBS.

Article Title: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on a Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradient from buffy coat preparations obtained from mice, and then washed twice with PBS.

Article Title: CD123 redirected multiple virus-specific T cells for acute myeloid leukemia.
Article Snippet: Hematopoietic stem cell transplantation (HSCT) has been increasingly used as a curative treatment for acute myeloid leukemia (AML).. However, relapse rates after HSCT in complete remission (CR) are reported between 30% and 70%.. In addition, numerous studies suggested that secondary viral infection from a variety of viruses including Epstein-Barr virus (EBV), adenovirus (Adv), and cytomegalovirus (CMV) are among the most common causes of death post-HSCT.

Article Title: Safety and feasibility of virus-specific T cells derived from umbilical cord blood in cord blood transplant recipients
Article Snippet: CBMCs were washed twice, resuspended in CellGenix media (CellGenix USA, Antioch, IL), and plated at approximately 1 × 10 7 cells per mL in 2 mL of dendritic cell (DC) medium (CellGenix media plus 2 mM L-glutamine) (GlutaMAX; Invitrogen, Carlsbad, CA) in a 6-well plate (Costar, Corning, NY) for 1 to 2 hours at 37°C in a humidified CO 2 incubator.

Article Title: CMV-Specific T-cells Generated From Naïve T-cells Recognize Atypical Epitopes And May Be Protective in Vivo
Article Snippet: CB was thawed and then purified by Ficoll (Lymphoprep; Nycomed) gradient separation.

Article Title: High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Article Snippet: Mononuclear cells were isolated from bone marrow aspirates (30 mL) by density gradient centrifugation in Ficoll (density, 1.077 g/mL) (Lymphoprep, Nycomed Pharma) and plated in noncoated 75–175 cm 2 polystyrene culture flasks (Corning Costar, Celbio, Italy) at a density of 160,000 cells/cm 2 .

Article Title: Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradients from buffy coat preparations obtained from mice, and then washed twice with PBS.

Incubation:

Article Title: Mesenchymal precursor cell
Article Snippet: Bone marrow mononuclear cells (BMMNC) were obtained by centrifugation over Ficoll 1.077 g/ml (Lymphoprep, Nycomed, Oslo, Norway) at 400 g for 30 minutes (min) and then washed and resuspended with Hank's buffered saline solution containing 1% bovine serum albumin and 10 mM HEPES, pH 7.35 (HBSS).

Article Title: Inhibition of TIM‐4 protects against cerebral ischaemia‐reperfusion injury
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on a Ficoll (Lymphoprep; PAA; Nycomed) gradient from buffy coat preparations obtained from mice and then washed twice in ice‐cold PBS.

Article Title: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on a Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradient from buffy coat preparations obtained from mice, and then washed twice with PBS.

Article Title: CD123 redirected multiple virus-specific T cells for acute myeloid leukemia.
Article Snippet: Hematopoietic stem cell transplantation (HSCT) has been increasingly used as a curative treatment for acute myeloid leukemia (AML).. However, relapse rates after HSCT in complete remission (CR) are reported between 30% and 70%.. In addition, numerous studies suggested that secondary viral infection from a variety of viruses including Epstein-Barr virus (EBV), adenovirus (Adv), and cytomegalovirus (CMV) are among the most common causes of death post-HSCT.

Article Title: Safety and feasibility of virus-specific T cells derived from umbilical cord blood in cord blood transplant recipients
Article Snippet: CBMCs were washed twice, resuspended in CellGenix media (CellGenix USA, Antioch, IL), and plated at approximately 1 × 10 7 cells per mL in 2 mL of dendritic cell (DC) medium (CellGenix media plus 2 mM L-glutamine) (GlutaMAX; Invitrogen, Carlsbad, CA) in a 6-well plate (Costar, Corning, NY) for 1 to 2 hours at 37°C in a humidified CO 2 incubator.

Article Title: CMV-Specific T-cells Generated From Naïve T-cells Recognize Atypical Epitopes And May Be Protective in Vivo
Article Snippet: CB was thawed and then purified by Ficoll (Lymphoprep; Nycomed) gradient separation.

Article Title: High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Article Snippet: Mononuclear cells were isolated from bone marrow aspirates (30 mL) by density gradient centrifugation in Ficoll (density, 1.077 g/mL) (Lymphoprep, Nycomed Pharma) and plated in noncoated 75–175 cm 2 polystyrene culture flasks (Corning Costar, Celbio, Italy) at a density of 160,000 cells/cm 2 .

Article Title: Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradients from buffy coat preparations obtained from mice, and then washed twice with PBS.

Bioprocessing:

Article Title: Mesenchymal precursor cell
Article Snippet: Bone marrow mononuclear cells (BMMNC) were obtained by centrifugation over Ficoll 1.077 g/ml (Lymphoprep, Nycomed, Oslo, Norway) at 400 g for 30 minutes (min) and then washed and resuspended with Hank's buffered saline solution containing 1% bovine serum albumin and 10 mM HEPES, pH 7.35 (HBSS).

Article Title: Inhibition of TIM‐4 protects against cerebral ischaemia‐reperfusion injury
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on a Ficoll (Lymphoprep; PAA; Nycomed) gradient from buffy coat preparations obtained from mice and then washed twice in ice‐cold PBS.

Article Title: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on a Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradient from buffy coat preparations obtained from mice, and then washed twice with PBS.

Article Title: CD123 redirected multiple virus-specific T cells for acute myeloid leukemia.
Article Snippet: Hematopoietic stem cell transplantation (HSCT) has been increasingly used as a curative treatment for acute myeloid leukemia (AML).. However, relapse rates after HSCT in complete remission (CR) are reported between 30% and 70%.. In addition, numerous studies suggested that secondary viral infection from a variety of viruses including Epstein-Barr virus (EBV), adenovirus (Adv), and cytomegalovirus (CMV) are among the most common causes of death post-HSCT.

Article Title: Safety and feasibility of virus-specific T cells derived from umbilical cord blood in cord blood transplant recipients
Article Snippet: CBMCs were washed twice, resuspended in CellGenix media (CellGenix USA, Antioch, IL), and plated at approximately 1 × 10 7 cells per mL in 2 mL of dendritic cell (DC) medium (CellGenix media plus 2 mM L-glutamine) (GlutaMAX; Invitrogen, Carlsbad, CA) in a 6-well plate (Costar, Corning, NY) for 1 to 2 hours at 37°C in a humidified CO 2 incubator.

Article Title: CMV-Specific T-cells Generated From Naïve T-cells Recognize Atypical Epitopes And May Be Protective in Vivo
Article Snippet: CB was thawed and then purified by Ficoll (Lymphoprep; Nycomed) gradient separation.

Article Title: High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Article Snippet: Mononuclear cells were isolated from bone marrow aspirates (30 mL) by density gradient centrifugation in Ficoll (density, 1.077 g/mL) (Lymphoprep, Nycomed Pharma) and plated in noncoated 75–175 cm 2 polystyrene culture flasks (Corning Costar, Celbio, Italy) at a density of 160,000 cells/cm 2 .

Article Title: Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradients from buffy coat preparations obtained from mice, and then washed twice with PBS.

Recombinase Polymerase Amplification:

Article Title: Mesenchymal precursor cell
Article Snippet: Bone marrow mononuclear cells (BMMNC) were obtained by centrifugation over Ficoll 1.077 g/ml (Lymphoprep, Nycomed, Oslo, Norway) at 400 g for 30 minutes (min) and then washed and resuspended with Hank's buffered saline solution containing 1% bovine serum albumin and 10 mM HEPES, pH 7.35 (HBSS).

Article Title: Inhibition of TIM‐4 protects against cerebral ischaemia‐reperfusion injury
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on a Ficoll (Lymphoprep; PAA; Nycomed) gradient from buffy coat preparations obtained from mice and then washed twice in ice‐cold PBS.

Article Title: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on a Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradient from buffy coat preparations obtained from mice, and then washed twice with PBS.

Article Title: CD123 redirected multiple virus-specific T cells for acute myeloid leukemia.
Article Snippet: Hematopoietic stem cell transplantation (HSCT) has been increasingly used as a curative treatment for acute myeloid leukemia (AML).. However, relapse rates after HSCT in complete remission (CR) are reported between 30% and 70%.. In addition, numerous studies suggested that secondary viral infection from a variety of viruses including Epstein-Barr virus (EBV), adenovirus (Adv), and cytomegalovirus (CMV) are among the most common causes of death post-HSCT.

Article Title: Safety and feasibility of virus-specific T cells derived from umbilical cord blood in cord blood transplant recipients
Article Snippet: CBMCs were washed twice, resuspended in CellGenix media (CellGenix USA, Antioch, IL), and plated at approximately 1 × 10 7 cells per mL in 2 mL of dendritic cell (DC) medium (CellGenix media plus 2 mM L-glutamine) (GlutaMAX; Invitrogen, Carlsbad, CA) in a 6-well plate (Costar, Corning, NY) for 1 to 2 hours at 37°C in a humidified CO 2 incubator.

Article Title: CMV-Specific T-cells Generated From Naïve T-cells Recognize Atypical Epitopes And May Be Protective in Vivo
Article Snippet: CB was thawed and then purified by Ficoll (Lymphoprep; Nycomed) gradient separation.

Article Title: High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Article Snippet: Mononuclear cells were isolated from bone marrow aspirates (30 mL) by density gradient centrifugation in Ficoll (density, 1.077 g/mL) (Lymphoprep, Nycomed Pharma) and plated in noncoated 75–175 cm 2 polystyrene culture flasks (Corning Costar, Celbio, Italy) at a density of 160,000 cells/cm 2 .

Article Title: Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradients from buffy coat preparations obtained from mice, and then washed twice with PBS.

Saline:

Article Title: Mesenchymal precursor cell
Article Snippet: Bone marrow mononuclear cells (BMMNC) were obtained by centrifugation over Ficoll 1.077 g/ml (Lymphoprep, Nycomed, Oslo, Norway) at 400 g for 30 minutes (min) and then washed and resuspended with Hank's buffered saline solution containing 1% bovine serum albumin and 10 mM HEPES, pH 7.35 (HBSS).

Article Title: Inhibition of TIM‐4 protects against cerebral ischaemia‐reperfusion injury
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on a Ficoll (Lymphoprep; PAA; Nycomed) gradient from buffy coat preparations obtained from mice and then washed twice in ice‐cold PBS.

Article Title: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on a Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradient from buffy coat preparations obtained from mice, and then washed twice with PBS.

Article Title: CD123 redirected multiple virus-specific T cells for acute myeloid leukemia.
Article Snippet: Hematopoietic stem cell transplantation (HSCT) has been increasingly used as a curative treatment for acute myeloid leukemia (AML).. However, relapse rates after HSCT in complete remission (CR) are reported between 30% and 70%.. In addition, numerous studies suggested that secondary viral infection from a variety of viruses including Epstein-Barr virus (EBV), adenovirus (Adv), and cytomegalovirus (CMV) are among the most common causes of death post-HSCT.

Article Title: Safety and feasibility of virus-specific T cells derived from umbilical cord blood in cord blood transplant recipients
Article Snippet: CBMCs were washed twice, resuspended in CellGenix media (CellGenix USA, Antioch, IL), and plated at approximately 1 × 10 7 cells per mL in 2 mL of dendritic cell (DC) medium (CellGenix media plus 2 mM L-glutamine) (GlutaMAX; Invitrogen, Carlsbad, CA) in a 6-well plate (Costar, Corning, NY) for 1 to 2 hours at 37°C in a humidified CO 2 incubator.

Article Title: CMV-Specific T-cells Generated From Naïve T-cells Recognize Atypical Epitopes And May Be Protective in Vivo
Article Snippet: CB was thawed and then purified by Ficoll (Lymphoprep; Nycomed) gradient separation.

Article Title: High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Article Snippet: Mononuclear cells were isolated from bone marrow aspirates (30 mL) by density gradient centrifugation in Ficoll (density, 1.077 g/mL) (Lymphoprep, Nycomed Pharma) and plated in noncoated 75–175 cm 2 polystyrene culture flasks (Corning Costar, Celbio, Italy) at a density of 160,000 cells/cm 2 .

Article Title: Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradients from buffy coat preparations obtained from mice, and then washed twice with PBS.

Gradient Centrifugation:

Article Title: Mesenchymal precursor cell
Article Snippet: Bone marrow mononuclear cells (BMMNC) were obtained by centrifugation over Ficoll 1.077 g/ml (Lymphoprep, Nycomed, Oslo, Norway) at 400 g for 30 minutes (min) and then washed and resuspended with Hank's buffered saline solution containing 1% bovine serum albumin and 10 mM HEPES, pH 7.35 (HBSS).

Article Title: Inhibition of TIM‐4 protects against cerebral ischaemia‐reperfusion injury
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on a Ficoll (Lymphoprep; PAA; Nycomed) gradient from buffy coat preparations obtained from mice and then washed twice in ice‐cold PBS.

Article Title: Inhibition of T cell immunoglobulin and mucin-1 (TIM-1) protects against cerebral ischemia-reperfusion injury
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on a Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradient from buffy coat preparations obtained from mice, and then washed twice with PBS.

Article Title: CD123 redirected multiple virus-specific T cells for acute myeloid leukemia.
Article Snippet: Hematopoietic stem cell transplantation (HSCT) has been increasingly used as a curative treatment for acute myeloid leukemia (AML).. However, relapse rates after HSCT in complete remission (CR) are reported between 30% and 70%.. In addition, numerous studies suggested that secondary viral infection from a variety of viruses including Epstein-Barr virus (EBV), adenovirus (Adv), and cytomegalovirus (CMV) are among the most common causes of death post-HSCT.

Article Title: Safety and feasibility of virus-specific T cells derived from umbilical cord blood in cord blood transplant recipients
Article Snippet: CBMCs were washed twice, resuspended in CellGenix media (CellGenix USA, Antioch, IL), and plated at approximately 1 × 10 7 cells per mL in 2 mL of dendritic cell (DC) medium (CellGenix media plus 2 mM L-glutamine) (GlutaMAX; Invitrogen, Carlsbad, CA) in a 6-well plate (Costar, Corning, NY) for 1 to 2 hours at 37°C in a humidified CO 2 incubator.

Article Title: CMV-Specific T-cells Generated From Naïve T-cells Recognize Atypical Epitopes And May Be Protective in Vivo
Article Snippet: CB was thawed and then purified by Ficoll (Lymphoprep; Nycomed) gradient separation.

Article Title: High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Article Snippet: Mononuclear cells were isolated from bone marrow aspirates (30 mL) by density gradient centrifugation in Ficoll (density, 1.077 g/mL) (Lymphoprep, Nycomed Pharma) and plated in noncoated 75–175 cm 2 polystyrene culture flasks (Corning Costar, Celbio, Italy) at a density of 160,000 cells/cm 2 .

Article Title: Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression
Article Snippet: Peripheral blood mononuclear cells were isolated by centrifugation on Ficoll (Lymphoprep, PAA, Nycomed, Oslo, Norway) gradients from buffy coat preparations obtained from mice, and then washed twice with PBS.



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